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wga staining solution w11261  (Thermo Fisher)


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    Structured Review

    Thermo Fisher wga staining solution w11261
    Wga Staining Solution W11261, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wga+staining+solution/wheat+germ+agglutinin/pm40158134-516-6-10
    Average 90 stars, based on 1 article reviews
    wga staining solution w11261 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Neurogenic Hypertension Mediated Mitochondrial Abnormality Leads to Cardiomyopathy: Contribution of UPR mt and Norepinephrine-miR- 18a-5p-HIF-1α Axis.
    Article Snippet: Heart cryosections were prepared using a CryoStarNX50 (Thermo Fisher Scientific, United States) and fixed in 4% paraformaldehyde solution for 30 min. .. Sections were washed 2 × 5 min in TBS post-fixation and then incubated with WGA staining solution (5 mg/ml, cat# W834, Thermo Fisher Scientific, United States) for 15 min at RT. .. Next, sections were washed and mounted with coverslip, and images were captured using a fluorescence microscope (Olympus IX71 Imaging Systems) to measured mean cardiomyocyte diameter and numbers per unit area.

    Article Title: Induction of Senescence by Loss of Gata4 in Cardiac Fibroblasts
    Article Snippet: After the heart sections were washed with PBS three times, they were incubated with AlexaFluor secondary antibodies (Thermofisher) for one hour at room temperature and mounted with Vectashield antifade mounting medium with DAPI (VectorLabs, Newark, CA, USA, cat# H-2000). tdTomato was visualized without immunostaining. .. For wheat germ agglutinin (WGA) staining, the heart sections were incubated with WGA staining solution (Thermofisher, cat# W32464) prepared per manufacturer’s instruction, along with secondary antibodies. .. Images were captured using Zeiss LSM 880 confocal microscope or Olympus IX81 epifluorescent microscope.

    Article Title: Induction of Senescence by Loss of Gata4 in Cardiac Fibroblasts.
    Article Snippet: After the heart sections were washed with PBS three times, they were incubated with AlexaFluor secondary antibodies (Thermofisher) for one hour at room temperature and mounted with Vectashield antifade mounting medium with DAPI (VectorLabs, Newark, CA, USA, cat# H-2000). tdTomato was visualized without immunostaining. .. For wheat germ agglutinin (WGA) staining, the heart sections were incubated with WGA staining solution (Thermofisher, cat# W32464) prepared per manufacturer’s instruction, along with secondary antibodies. .. Images were captured using Zeiss LSM 880 confocal microscope or Olympus IX81 epifluorescent microscope.

    Whole Genome Amplification:

    Article Title: Neurogenic Hypertension Mediated Mitochondrial Abnormality Leads to Cardiomyopathy: Contribution of UPR mt and Norepinephrine-miR- 18a-5p-HIF-1α Axis.
    Article Snippet: Heart cryosections were prepared using a CryoStarNX50 (Thermo Fisher Scientific, United States) and fixed in 4% paraformaldehyde solution for 30 min. .. Sections were washed 2 × 5 min in TBS post-fixation and then incubated with WGA staining solution (5 mg/ml, cat# W834, Thermo Fisher Scientific, United States) for 15 min at RT. .. Next, sections were washed and mounted with coverslip, and images were captured using a fluorescence microscope (Olympus IX71 Imaging Systems) to measured mean cardiomyocyte diameter and numbers per unit area.

    Article Title: VPO1 Promotes Programmed Necrosis of Cardiomyocytes in Rats with Chronic Heart Failure by Upregulating CYLD.
    Article Snippet: .. Sections were then reacted with TYP-570 (AWI0693a, Abiowell) and stained with WGA staining solution (W21405, Thermo Fisher Scientific). ..

    Article Title: Induction of Senescence by Loss of Gata4 in Cardiac Fibroblasts
    Article Snippet: After the heart sections were washed with PBS three times, they were incubated with AlexaFluor secondary antibodies (Thermofisher) for one hour at room temperature and mounted with Vectashield antifade mounting medium with DAPI (VectorLabs, Newark, CA, USA, cat# H-2000). tdTomato was visualized without immunostaining. .. For wheat germ agglutinin (WGA) staining, the heart sections were incubated with WGA staining solution (Thermofisher, cat# W32464) prepared per manufacturer’s instruction, along with secondary antibodies. .. Images were captured using Zeiss LSM 880 confocal microscope or Olympus IX81 epifluorescent microscope.

    Article Title: Induction of Senescence by Loss of Gata4 in Cardiac Fibroblasts.
    Article Snippet: After the heart sections were washed with PBS three times, they were incubated with AlexaFluor secondary antibodies (Thermofisher) for one hour at room temperature and mounted with Vectashield antifade mounting medium with DAPI (VectorLabs, Newark, CA, USA, cat# H-2000). tdTomato was visualized without immunostaining. .. For wheat germ agglutinin (WGA) staining, the heart sections were incubated with WGA staining solution (Thermofisher, cat# W32464) prepared per manufacturer’s instruction, along with secondary antibodies. .. Images were captured using Zeiss LSM 880 confocal microscope or Olympus IX81 epifluorescent microscope.

    Article Title: A Helical Polypeptide‐Based Potassium Ionophore Induces Endoplasmic Reticulum Stress‐Mediated Apoptosis by Perturbing Ion Homeostasis
    Article Snippet: .. In the following step, the cells were fixed by a 4% paraformaldehyde solution for 10 min. Alexa Fluor 555 conjugate of the WGA staining solution (5 μg mL −1 in PBS, ThermoFisher Scientific, USA) was added to each well for 5 min., and then, the cells were washed with PBS three times prior to DAPI staining (300 × 10 −9 m ). .. The cell images were obtained by CLSM (LSM 800 META, ZEISS, Germany).

    Staining:

    Article Title: Neurogenic Hypertension Mediated Mitochondrial Abnormality Leads to Cardiomyopathy: Contribution of UPR mt and Norepinephrine-miR- 18a-5p-HIF-1α Axis.
    Article Snippet: Heart cryosections were prepared using a CryoStarNX50 (Thermo Fisher Scientific, United States) and fixed in 4% paraformaldehyde solution for 30 min. .. Sections were washed 2 × 5 min in TBS post-fixation and then incubated with WGA staining solution (5 mg/ml, cat# W834, Thermo Fisher Scientific, United States) for 15 min at RT. .. Next, sections were washed and mounted with coverslip, and images were captured using a fluorescence microscope (Olympus IX71 Imaging Systems) to measured mean cardiomyocyte diameter and numbers per unit area.

    Article Title: VPO1 Promotes Programmed Necrosis of Cardiomyocytes in Rats with Chronic Heart Failure by Upregulating CYLD.
    Article Snippet: .. Sections were then reacted with TYP-570 (AWI0693a, Abiowell) and stained with WGA staining solution (W21405, Thermo Fisher Scientific). ..

    Article Title: Induction of Senescence by Loss of Gata4 in Cardiac Fibroblasts
    Article Snippet: After the heart sections were washed with PBS three times, they were incubated with AlexaFluor secondary antibodies (Thermofisher) for one hour at room temperature and mounted with Vectashield antifade mounting medium with DAPI (VectorLabs, Newark, CA, USA, cat# H-2000). tdTomato was visualized without immunostaining. .. For wheat germ agglutinin (WGA) staining, the heart sections were incubated with WGA staining solution (Thermofisher, cat# W32464) prepared per manufacturer’s instruction, along with secondary antibodies. .. Images were captured using Zeiss LSM 880 confocal microscope or Olympus IX81 epifluorescent microscope.

    Article Title: Induction of Senescence by Loss of Gata4 in Cardiac Fibroblasts.
    Article Snippet: After the heart sections were washed with PBS three times, they were incubated with AlexaFluor secondary antibodies (Thermofisher) for one hour at room temperature and mounted with Vectashield antifade mounting medium with DAPI (VectorLabs, Newark, CA, USA, cat# H-2000). tdTomato was visualized without immunostaining. .. For wheat germ agglutinin (WGA) staining, the heart sections were incubated with WGA staining solution (Thermofisher, cat# W32464) prepared per manufacturer’s instruction, along with secondary antibodies. .. Images were captured using Zeiss LSM 880 confocal microscope or Olympus IX81 epifluorescent microscope.

    Article Title: A Helical Polypeptide‐Based Potassium Ionophore Induces Endoplasmic Reticulum Stress‐Mediated Apoptosis by Perturbing Ion Homeostasis
    Article Snippet: .. In the following step, the cells were fixed by a 4% paraformaldehyde solution for 10 min. Alexa Fluor 555 conjugate of the WGA staining solution (5 μg mL −1 in PBS, ThermoFisher Scientific, USA) was added to each well for 5 min., and then, the cells were washed with PBS three times prior to DAPI staining (300 × 10 −9 m ). .. The cell images were obtained by CLSM (LSM 800 META, ZEISS, Germany).



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    a Cell type-specific tissue preparation for the Deep Visual Proteomics (DVP) spatial proteomics pipeline, starting with FFPE tissue sectioning, mounting, staining, and image acquisition. b Representative images of WGA-stained lymph node tissue, showing one raw, one segmented, and one classified image (lymphocytes in pink, SR cells in green and segmentation artifacts in blue). c Export mask of classified SR cells. d Illustration of the semi-automated laser microdissection sample collection, and processing, followed by ( e ) the liquid chromatography-mass spectrometry (LC-MS) setup. Illustrated with BioRender.

    Journal: NPJ Precision Oncology

    Article Title: Deep visual proteomics reveals DNA replication stress as a hallmark of signet ring cell carcinoma

    doi: 10.1038/s41698-025-00819-7

    Figure Lengend Snippet: a Cell type-specific tissue preparation for the Deep Visual Proteomics (DVP) spatial proteomics pipeline, starting with FFPE tissue sectioning, mounting, staining, and image acquisition. b Representative images of WGA-stained lymph node tissue, showing one raw, one segmented, and one classified image (lymphocytes in pink, SR cells in green and segmentation artifacts in blue). c Export mask of classified SR cells. d Illustration of the semi-automated laser microdissection sample collection, and processing, followed by ( e ) the liquid chromatography-mass spectrometry (LC-MS) setup. Illustrated with BioRender.

    Article Snippet: For Wheat Germ Agglutinin (WGA) labeling, sections on membrane PEN slides were incubated with WGA staining solution (Biotium, 29023; diluted 1:1000) in a light-protected environment at 37 °C for 10 min. For pan-cytokeratin (CK), CD8, PD1, PDL1 and pATR staining, antigen retrieval was achieved by immersing the tissue sections on glass slides in EDTA buffer (Sigma, E1161; pH 8.5) at 90 °C for 30 min.

    Techniques: Staining, Laser Capture Microdissection, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy